Characterization of DNA Layers Adsorbed on Glassy Carbon Electrodes |
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Authors: | María L. Pedano Lía I. Pietrasanta Manuel López Teijelo Gustavo A. Rivas |
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Affiliation: | 1. INFIQC, Departamento de Físico Química, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina;2. C.M.A., Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina |
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Abstract: | A DNA layer adsorbed at glassy carbon electrodes (GCE) was characterized by ellipsometry, atomic force microscopy (AFM) and scanning electron microscopy (SEM). The presence of the adsorbed DNA layer on polished glassy carbon electrodes was assessed indirectly by ellipsometric measurements. Ellipsometry was also useful to evaluate the influence of the oxide layer formed on glassy carbon electrodes, either spontaneously or after electrochemical pretreatments, on the DNA adsorption and further electrooxidation process. SEM and AFM images of the electrode surface covered by a thick layer of DNA reveal a nonuniform distribution, leaving channels and islands of the biological material. |
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Keywords: | DNA adsorption DNA‐modified electrode Glassy carbon electrode Ellipsometry AFM SEM |
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