Building‐Block Approach for the Straightforward Incorporation of a New FRET (Fluorescence‐Resonance‐Energy Transfer) System into Synthetic DNA |
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Authors: | Lilia Clima Willi Bannwarth |
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Institution: | 1. Institut für Organische Chemie und Biochemie, Albert‐Ludwigs Universit?t Freiburg, Albertstr. 21, D‐79104 Freiburg, (phone: +497612036073;2. fax: +497612038705) |
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Abstract: | The synthesis of the two new phosphoramidites 5 and 8 bearing a carbostyril (=quinolin‐2(1H)‐one) chromophore used as donor entity in our recently developed new FRET (fluorescence‐resonance‐energy transfer) system is described (Schemes 1 and 2) The high stability of the chromophore to basic conditions enables the incorporation of the phosphoramidites directly into DNA during solid‐phase synthesis (Schemes 3 and 4). Since this is also possible for the (bathophenanthroline)ruthenium(II) complex used as acceptor (Scheme 4, Steps d and e), the whole labelling procedure to insert the FRET system into synthetic DNA is straightforward and represents a major improvement to our previous strategy. |
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Keywords: | Fluorescence‐resonance‐energy transfer (FRET) Ruthenium complexes DNA Phosphoramidites Bathophenanthroline complexes |
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