Direct Electrochemistry of Hemoglobin and Its Electrocatalysis Based on Hyaluronic Acid and Room Temperature Ionic Liquid |
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| Authors: | Ruifang Gao Xiaodong Shangguan Guangjun Qiao Jianbin Zheng |
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| Institution: | Institute of Analytical Science, Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi, 710069, P.?R. China |
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| Abstract: | A biocomposite material of hyaluronic acid (HA) and room temperature ionic liquid, 1‐butyl‐3‐methyl‐imidazolium tetrafluoroborate (BMIMBF4) was fabricated and characterized by voltammetry and electrochemical impedance spectroscopy. Hemoglobin (Hb), as a model protein, was immobilized in this biocomposite on glassy carbon electrode to investigate its direct electrochemistry. A pair of well‐defined, quasireversible cyclic voltammetric peaks appeared with the formal potentials (E°′) at ?0.360 V (vs. SCE). The Hb modified electrode exhibited an excellent electrocatalytic activity to the reduction of H2O2 with a wide linear range, good selectivity, sensitivity and reproducibility, which had potential application to fabrication the third‐generation biosensor. |
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| Keywords: | Electroanalysis Direct electrochemistry Homoglobin Room temperature ionic liquids Hyaluronic acid |
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