Synthesis of pyrazolo[3,4‐b]pyridines and attachment of amino acids and carbohydrate as linkers

Synthesis of novel pyrazolo[3,4‐b]pyridines has been achieved successfully by sequence of Gould ‐ Jacobs reaction between 5‐aminopyrazole and diethylethoxymethylenemalonate in good yield. Further the pyrazolo[3,4‐b]pyridines were converted into succinimidoyl active esters which are then replaced by biological samples such as amino acids and carbohydrate in slightly aqueous medium.