Electrochemistry of Cytochrome P450 2B6 on Electrodes Modified with Zirconium Dioxide Nanoparticles and Platin Components |
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Authors: | Lei Peng Xiaodi Yang Qianqian Zhang Songqin Liu |
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Affiliation: | 1. School of Chemistry and Environmental Science, Nanjing Normal University, Nanjing, 210097, P.?R. China;2. School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 210096, P.?R. China |
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Abstract: | The direct electrochemical and electrocatalytic behavior of the immobilized cytochrome P450 2B6 (CYP2B6) on zirconium dioxide nanoparticles (ZrO2) was investigated. The film of nano‐structured ZrO2 that incorporated cytochrome P450 2B6 (CYP2B6) with colloidal paltin, which was stabilized by poly‐lysine (Pt‐PLL), was prepared on glassy carbon electrodes. In anaerobic solutions, the immobilized CYP2B6 exhibited a reversible electron transfer between the heme electroactive center of CYP2B6 and electrodes with a formal potential of ?(0.449±0.004) V at pH 7.4. In air‐saturated solutions, an increased bioelectrocatalytic reduction current could be obtained with the CYP2B6‐modified electrode with the addition of anticancer drugs, such as lidocaine. This leads to the construction of disposable biosensors for drugs by utilizing the electrochemical activity and catalytic reactions of the immobilized CYP2B6. |
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Keywords: | Cytochrome P450 Zirconium dioxide nanoparticles Lidocaine Biosensor Electrocatalytic behavior |
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