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Generation of Nanopores Down to 10 nm for Use in Deep‐Nulling Interferometry
Authors:Axel Wehling Dr.  Wiebke H. Pohl  Birgit Gerke  Stephan Kipp Dr.  Peter J. Walla Prof. Dr.
Affiliation:1. Department of Biophysical Chemistry, Institute for Physical and Theoretical Chemistry, University of Braunschweig, 38106 Braunschweig, Germany, Fax: (+49)?531‐391‐5352;2. Department of Spectroscopy and Photochemical Kinetics, Max‐Planck‐Institute for Biophysical Chemistry, 37077 G?ttingen, Germany
Abstract:Scanning electron microscope images show that it is easy to generate nanopores on polycarbonate membranes with well‐defined pore diameters by ion‐track perforation and subsequent magnetron sputtering with metal. The size reduction of the nanopores during sputtering with gold is a linear function of time. Images of different angles and from the bottom side of the membrane show that the channels are the smallest very close to the surface of the metal layer, have a conelike shape, and reach about half as much into the polymer membranes as the metal‐layer thickness. This topographical pore shape is ideal for use as optically coherent near‐field sources in deep‐nulling microscopy. We present the first results of significantly improved nulling stabilization in the presence (<2 nm optical pathway difference) and the absence (<0.6 nm optical pathway difference) of the nanoapertures in the focal region of a deep‐nulling microscope.
Keywords:electron microscopy  interferometry  label‐free  nanopores  single‐molecule studies
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