基于近红外光谱分析的脊髓损伤后神经细胞活性检测方法

交通意外、高空跌落、机械撞击等事故，可能导致人体脊髓损伤，从而中断了人体神经信号的传输信道，致使部分肢体功能的丧失。由于目前没有脊髓受损部位神经细胞活性检测的方法，而常规的检测仪器如X射线、CT等传统仪器不能提供神经细胞活性的任何信息，医生无法对患者病情有更清晰的了解，这就可能延误最佳的治疗时机，致使患者终生瘫痪。光谱学方法可以检测细胞组织的改变，且近红外光谱技术是一种测量速度快，操作简便的无损测量技术。所以，针对现有医学检测设备的不足，在动物实验的基础上，根据物质在近红外光波段的吸光特性，并结合近红外光谱分析技术的定性判别和定量分析的特性，通过对脊髓损伤后神经细胞活性的检测，采用聚类算法对脊髓损伤部位的神经特异性核蛋白和神经递质进行分类和仿真，并采用偏最小二乘算法计算其含量，实现了脊髓损伤部位神经细胞活性的精确检测。该方法为脊髓损伤后神经细胞活性的检测提供理论依据，给患者带来肢体功能重建和康复的希望，为临床医学提供了一种无创检测神经细胞活性的方法。

Neural Cell Activity Detection Method Based on Near Infrared Spectroscopy Analysis of Spinal Cord Injury

Traffic accidents,high-altitude drops,mechani cal shock and other accidents may lead to human spinal cord injury,interrupting the transmission channel of human nerve signals,resulting in loss of some limb function.Since there is no method of spinal cord injury site of nerve cell act ivity,while the conventional testing instruments such as X ray,CT and other tr aditional instruments cannot provide any nerve cell activity,doctors couldn't have a clearer understanding of the patient's condition,which may delay the be s t timing of treatment,thus causing lifelong paralysis.Spectroscopy method can be used in the detection of cell organization change while near infrared spectru m technology is a kind of fast measurement and simple operation of nondestructiv e measuring.Therefore,aiming at the shortcomings of the existing medical testi ng equipment,on the basis of animal experiment,this paper uses near infrared s pectrum technology,based on the absorption characteristics of the material in n ear infrared wave band,and combined with near infrared spectrum analysis techni que of qualitative determination and quantitative analysis of the characteristic s.Using clustering algorithm for spinal cord injury site of neuron-specific nu clear protein and neurotransmitters classify and simulation,partial least squar es (PLS) algorithm is used to calculate the content to achieve a spinal cord acc urate detection of the site of injury nerve cell activity.This method provides theoretical basis for the detection of spinal cord injury,bringing hopes to pat ients with limb function reconstruction and rehabilitation,for clinical provide s a method to detect the activity of nerve cells in the noninvasive.