水体细菌微生物多波长散射光谱快速定量检测

通过分析细菌细胞的结构特征，将细菌菌体的多波长散射分为外部结构散射和内部结构散射两个部分，建立了细菌菌体前向散射光谱解释模型。利用该模型对大肠杆菌400～900 nm波段的前向散射光进行了快速解析，得到了大肠杆菌外部结构、内部结构的平均粒径大小及两结构占细菌体前向散射的比例；基于单细胞的散射光密度与整体细菌悬浮液光密度之间的关系可以快速检测出细菌的浓度。多次细菌浓度测量结果之间的最大差异为1.83%，且与平板法相比较，测量结果在同一量级，相对误差为3.44%。对不同生长时期的大肠杆菌和肺炎克雷伯菌进行了光谱解析，得到了两种细菌浓度及菌体大小随时间的变化曲线。研究结果不仅为细菌微生物生长过程的科学研究提供了一种快捷方法，而且为水体细菌微生物的快速检测与预警提供了技术手段。

Rapid Quantitative Detection of Bacterial in Water Based on Multi-Wave length Scattering Spectra

The structures of bacterial cells are analyzed in this paper.The scattering components of individual cell were divided into two parts including external structure and internal structure.The interpretation model of bacteria about scattering light is established.The model is used to analyze the scattering light of Escherichia coli in the region of 400~900 nm.The average size of external structure and the internal structure can be obtained,and the ratio of the two parts is also obtained.According to the relationship of the optical density of single cell and the overall measurement,the concentration of bacterial can be obtained quickly.The maximum difference in all the concentrations of the bacteria repeated measurements is 1.83%;compared with the plate culture method,the measurement results were in the same order of magnitude,with relative error of 3.43%.The scattering light of Escherichia coli and Klebsiella pneumoniae are analyzed in different growth stages,the curves of the concentration and the size of the two species bacteria over time are obtained.The results can provide a quick way for the study of bacterial growth and technical support for rapid detection of bacteria in the water.