基于2D-COS红外光谱的附子炮制过程时序段解析研究

采用傅里叶变换红外光谱技术，获得蒸制过程中附子红外光谱及二维相关谱(Tow-dimensional correlation, 2D-COS)，研究附子炮制过程特征。分别比较原始红外光谱、二阶导数谱及二维相关谱，结果表明红外原始光谱图十分相似，结合二阶导数光谱分析1 634.87 cm-1为羰基峰；1 603.18，1 571.58，1 485.09和1 413.08 cm-1为苯环的骨架振动峰，851.41和759.24 cm-1 为苯环C-H键的振动峰；1 153.73，1 081.84和1 021.35 cm-1为生物碱中酯键和醇羟基的特征峰。二维相关谱结果揭示附子在炮制过程中发生的2个过程时序段，分别为2～3和8～9 h。研究数据有助于临床根据适应证选择适宜的煎煮时间，避免煎煮不足导致中毒或煎煮太过影响疗效。

Research on Steaming Procedure of Aconite Root with FTIR and Two-Dimensional IR Correlation Spectroscopy

Fourier transform infrared spectroscopy (FTIR),second derivative infrared spectroscopy,and two-dimensional correlation infrared spectroscopy (2D-IR) were used to study the steaming procedure of aconite tuber.The results showed that samples with steaming procedures were similar to each other in one-dimensional IR spectra but different in 2D-IR spectra.The FTIR spectra combined with second derivative infrared spectroscopy of different samples during boiling procedure present characteristic peaks at 1 634.87 cm-1 (C=O),1 603.18,1 571.58 cm-1,1 485.09,1 413.08 cm-1 (vibration of phenyl framework) and 1 153.73,1 081.84,1 021.35 cm-1 (C-O) respectively.There were two conspicuous time sequences in two dimensional infrared correlation spectroscopy of autopeaks intensity.Since,the intensity of autopeaks was relatively high during 2～3 and 8～9 h.The function of FTIR and 2D-IR for analysis of the alkaloid from aconite root attributed high speed and high efficiency.The results were reliable and valuable.