| 基于碳纳米管修饰电极的胆碱电化学发光生物传感器研制 |
| |
| 引用本文： | 赵金金,吴梅笙,屠一锋.基于碳纳米管修饰电极的胆碱电化学发光生物传感器研制[J].分析化学,2011,39(7). |
| |
| 作者姓名： | 赵金金 吴梅笙 屠一锋 |
| |
| 作者单位： | 苏州大学化学系苏州工业园区独墅湖高教区; |
| |
| 基金项目： | 国家自然科学基金(Nos.20275025,20675055)资助 |
| |
| 摘 要： | 在碳纳米管(CNTs)和K3Fe(CN)6修饰的铂电极上吸附固定胆碱氧化酶,以鲁米诺为发光试剂,研制了胆碱电化学发光(ECL)生物传感器。CNTs可有效提高电极表面的电荷传输能力、提高电极表面的生物相容性和对酶分子的固载能力;K3Fe(CN)6对酶活性具有激活作用,同时对H2O2增敏的鲁米诺ECL有增强作用,均有利于提高传感器的检测灵敏度。研究表明,将CNTs分散液与K3Fe(CN)6混合,滴涂修饰在Pt电极上,吸附固定胆碱氧化酶,制备传感器。此传感器在含有8×10-6mol/L鲁米诺的磷酸盐缓冲液(pH7.4)、30℃条件下产生的ECL强度与胆碱浓度在1×10-7～4×10-3mol/L范围内呈线性关系,相关系数为0.994,检出限为1.2×10-8 mol/L。此生物传感器应用于鼠血样中胆碱的测定,测得结果为2.68 mg/L,平均回收率为101.1%。传感器具有快速、稳定和重现性好等特点,有望应用于常规分析。
|
| 关 键 词： | 电化学发光 生物传感器 鲁米诺 胆碱 碳纳米管 |
Development of Electrochemiluminescent Biosensor for Choline Based on Carbon Nanotubes Modified Electrode |
| |
| ZHAO Jin-Jin,WU Mei-Sheng,TU Yi-Feng.Development of Electrochemiluminescent Biosensor for Choline Based on Carbon Nanotubes Modified Electrode[J].Chinese Journal of Analytical Chemistry,2011,39(7). |
| |
| Authors: | ZHAO Jin-Jin WU Mei-Sheng TU Yi-Feng |
| |
| Institution: | ZHAO Jin-Jin,WU Mei-Sheng,TU Yi-Feng(Department of Chemistry,Soochow University,Dushu Lake Higher Education Town,Industrial Park,Suzhou 215123) |
| |
| Abstract: | An electrochemiluminescent(ECL) choline biosensor was developed by drop-coating of choline oxidase(ChOx) onto a carbon nanotubes(CNTs)/potassium ferricyanide modified platinum electrode with ECL of luminol as readout signal.Due to the improvement of biocompatibility and electron transfer of electrode surface from CNTs,meanwhile the activation for enzyme and the ECL emission from K3Fe(CN)6,the developed biosensor possesses excellent analytical properties.The biosensor gives optimal results while the Pt elect... |
| |
| Keywords: | Electrochemiluminescence Biosensor Luminol Choline Carbon nanotubes |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
