| 互补多酶解法在蛋白质C末端质谱检测中的应用 |
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| 引用本文： | 杨洁,姚树森,赵永强,薛燕,李萍.互补多酶解法在蛋白质C末端质谱检测中的应用[J].分析化学,2011,39(4):486-490. |
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| 作者姓名： | 杨洁 姚树森 赵永强 薛燕 李萍 |
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| 作者单位： | 解放军总医院药品保障中心;解放军疾病预防控制中心办公室;国家生物医学分析中心; |
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| 基金项目： | 科技重大专项-重大新药创制项目(No.2009ZX09501-031)资助 |
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| 摘 要： | 建立了互补型多酶解法与串联质谱联用鉴定蛋白C末端技术.在大量蛋白的实际检测中,根据蛋白序列分别采用溴化氰、胰蛋白酶、谷氨酸内切酶和糜蛋白酶进行酶解或混合酶解.利用此技术对8个蛋白不同长度的C末端肽段(分子量分布在200～3000 Da之间,目的肽段分别为m/z 272.20,788.45,796.48,944.58,1...
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| 关 键 词： | 多酶解方法 电喷雾串联质谱技术 C末端测序 |
Application of Complementary Multi-enzyme Digestion for Protein C-terminal by Mass Spectrometry Sequencing |
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| YANG Jie,YAO Shu-Sen,ZHAO Yong-Qiang,XUE Yan,LI Ping.Application of Complementary Multi-enzyme Digestion for Protein C-terminal by Mass Spectrometry Sequencing[J].Chinese Journal of Analytical Chemistry,2011,39(4):486-490. |
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| Authors: | YANG Jie YAO Shu-Sen ZHAO Yong-Qiang XUE Yan LI Ping |
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| Institution: | 1(Department of Pharmacy,Chinese People′s Liberty Army,General Hospital,Beijing 100853)2(Chinese People′s Liberty Army,Center Office for Disease Control and Prevention,Beijing 100850)3(National Center of Biomedical Analysis,Beijing 100850) |
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| Abstract: | According to protein sequence,the cyanogen bromide,trypsin,Glu-C and chymotrypsin or mixing enzymes were used for sequencing of protein C-terminal by MS/MS.Utilizing this technology sequencing of 8 proteins with different length C-terminal,molecular weight range from 200 to 3000 Da and m/z of 272.20,788.45,796.48,944.58,1146.69,1151.67,1529.61 and 2147.04 were processed by ESI-MS/MS detection.Different length C-terminal amino acid sequence of 8 proteins: PR,SLSLSPGK,GNVTGMFA,LACGVIGIAQ,DHWPEANQVG,FIAWLVKGRG... |
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| Keywords: | Multi-enzymatic digestion method Electrospray ionization mass spectrometry/mass spectrometry C-terminal sequencing |
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