肉食品中常见4种抗生素残留的高效液相色谱分析

建立了同时测定肉食品中泰乐菌素、替米考星、氯霉素、氟苯尼考的高效液相色谱方法，样品经乙腈提取，正己烷脱脂， HLB小柱净化后，以C18反相色谱柱为分离柱，甲醇－磷酸二氢钠缓冲溶液(pH 3.0,含体积比为10％的甲醇溶液）为流动相进行梯度洗脱，检测波长λ为275 nm.泰乐菌素、替米考星、氯霉素、氟苯尼考的线性范围是0.1～20.0 mg/L，相关系数分别为0.9987、0.9992、0.9985、0.9970.其平均回收率为75％～87%，相对标准偏差为1.35％～5.41％，泰乐菌素、替米考星、氯霉素、氟苯尼考的检出限分别为20、32、19、16μg/kg.方法满足肉食品中泰乐菌素、替米考星、氯霉素、氟苯尼考的残留量测定.

Simultaneous Determination of Macrolide and Chloramphenicols Antibiotic Residues in Animal Derived Food by HPLC Method

An HPLC method for the simultaneous determination of residues of tylosin(TYL),tilmicosin(TIL),chloramphenicol(CAP) and florfenicol(FF) in animal-derived food was developed.The muscle tissue samples were extracted by acetonitrile,then defatted with hexane,and cleaned up on a HLB SPE column.The analytes were separated on a reversed-phase C18 column with the methanol-20 mmol/L NaH2PO4 solution(pH=3.0,containing 10% methanol),the UV detector was set at λ=275 nm.The standard curves were linear in the range of 0.1～20.0 mg/L for all analytes with correlation coefficients of 0.9970～0.9992.The detection limits for tylosin,tilmicosin,chloramphenicol(CAP),and florfenicol(FF),were 20 μg/kg,32 μg/kg,19 μg/kg,16 μg/kg,respectively.The average recoveries of four analytes ranged from 75% to 87%.The relative standard deviations were between 1.35%～5.41%.The method can meet the requirement for the simultaneously determination of tylosin,tilmicosin,chloramphenicol(CAP),and florfenicol(FF) in beef products.