| 发酵过程中菌液浓度的检测 |
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| 引用本文： | 杨帆,李淑梅,陈萍,李进芬.发酵过程中菌液浓度的检测[J].光谱实验室,2009,26(6):1643-1645. |
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| 作者姓名： | 杨帆 李淑梅 陈萍 李进芬 |
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| 作者单位： | 杨帆,陈萍,李进芬(新乡医学院微生物学教研室,河南省新乡市华兰大道东段,453003);李淑梅(河南科技学院化学化工学院,河南省新乡市华兰大道东段,453003) |
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| 摘 要： | 采用分光光度测量发酵菌液浓度,选择波长215nm,首先测定样品中菌液的吸光度,再根据校准曲线获得菌体浓度。该法设备简单,操作方便、快速,测量相对误差小于3%,相对标准偏差小于2%,精密度高,准确度好,适合于生产监控。
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| 关 键 词： | 分光光度法 菌液浓度 |
Measurement of Cell Concentration in Fermentation Liquid |
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| YANG Fan LI Shu-Meia CHEN Ping LI Jin-Fen,a.Measurement of Cell Concentration in Fermentation Liquid[J].Chinese Journal of Spectroscopy Laboratory,2009,26(6):1643-1645. |
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| Authors: | YANG Fan LI Shu-Meia CHEN Ping LI Jin-Fen a |
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| Institution: | YANG Fan LI Shu-Meia CHEN Ping LI Jin-Fen (Department of Microbiology,Xinxiang Medical College,Xinxiang,Henan 453003,P.R.China) a(School of Chemistry and Chemical Engineering,Henan Institute of Science and Technology,P.R.China) |
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| Abstract: | The cell concentration in fermentation liquid was determined by spectrophotometry at 215nm.According to the linear relationship between the cell concentration and its absorption in some range,the cell concentration was obtained.This method is simple,easy,quick,and accurate,and is adaptable to control practical production. |
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| Keywords: | Spectrophotometry Cell Concentration |
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