| 毛细管气相色谱法同时测定鸡蛋中的胆固醇和α-维生素E |
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| 引用本文： | 金米聪,王立,符展明.毛细管气相色谱法同时测定鸡蛋中的胆固醇和α-维生素E[J].色谱,2001,19(5):478-480. |
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| 作者姓名： | 金米聪 王立 符展明 |
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| 作者单位： | 宁波市卫生防疫站 |
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| 摘 要： | 采用 2 0mol/L的KOH乙醇溶液直接皂化 ,石油醚萃取 ,大口径毛细管气相色谱法测定鸡蛋中的胆固醇和α 维生素E(α VE) ,讨论并确定了鸡蛋样品皂化的最佳条件。胆固醇和α VE的回收率分别为 91.0 %～ 93.3%和82 .5 %～ 95 .0 % ,其RSD分别为 2 7%～ 3 1%和 4 9%～ 6 9% (n =5 )。该法具有简便、快速、灵敏、准确等优点。
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| 关 键 词： | 毛细管气相色谱法 胆固醇 α维生素E 鸡蛋 |
| 文章编号： | 1000-8713(2001)05-0478-03 |
| 修稿时间： | 2000年12月8日 |
Determination of cholesterol and alpha-tocopherol in eggs by capillary gas chromatography] |
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| JIN Mi cong,WANG Li,FU Zhan ming.Determination of cholesterol and alpha-tocopherol in eggs by capillary gas chromatography][J].Chinese Journal of Chromatography,2001,19(5):478-480. |
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| Authors: | JIN Mi cong WANG Li FU Zhan ming |
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| Institution: | Ningbo Sanitation and Antiepidemic Station, Ningbo 315010, China. jmcjc@163.com |
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| Abstract: | A simple and rapid method has been developed for simultaneous determination of cholesterol and alpha-tocopherol in eggs. The method involves alcoholic KOH saponification of the samples, extraction with petroleum ether, and determination by wide-bore capillary gas chromatography. The recoveries of cholesterol and alpha-VE in eggs were 91.0%-93.3% and 82.5%-95.0%, with RSDs of 2.7%-3.1% and 4.9%-6.9% respectively. |
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| Keywords: | NULL |
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