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Biomimetic Synthesis of Silver Nanoparticle Using Leaf Extract of Mangifera Indica and Their Antibacterial Efficacy
Authors:Vikas Sarsar  Manjit K.Selwal  Krishan K.Selwal
Affiliation:1. Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, India2. Department of Animal Sciences, The Ohio State University, Columbus (Ohio), USA
Abstract:The aim of this study to focused on bioinspired synthesis of silver nanoparticles (AgNPs) as a viable alternative to eradicate the existing physicochemical processes. In this context, the bioinspired AgNPs were synthesized using leaf extract of M. indica. Optimization of the experimental conditions for the rapid and high yield of AgNPs in minimum investment of time and expense have been carried out along with their antibacterial efficacy evaluated. For this reason, the variation of parameters like the concentration of the silver precursors, reducing agent, time, pH, and temperature of synthesis were realized. Synthesized AgNPs were characterized by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) techniques. UV-Visible spectra gave surface plasmon resonance (SPR) at 440 nm for AgNPs. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) techniques were further confirmed the synthesis and crystalline nature of AgNPs respectively. Transmission electron microscopy (TEM) observed spherical shapes of synthesized AgNPs within range 5~20 nm. The results of the current study indicate that optimization process play a pivotal role in the AgNPs synthesis and biogenic synthesized AgNPs might be used against bacterial pathogens.
Keywords:AgNPs  Antibacterial efficacy  FTIR  TEM  XRD  
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