激光诱导植物荧光寿命校正技术及其特性分析

激光诱导植物荧光寿命测量法是在植物荧光光谱分析法基础上开发的一种评估植物生长状况及环境监测的新技术。根据植物叶绿素荧光信号的物理特性，利用信息仿真技术开发了一种叶绿素荧光寿命校正方法，可提高植物叶绿素荧光寿命的测量精度。利用激光诱导叶绿素荧光寿命测量系统分别测得叶绿素荧光及其背景信号，先用解卷积法叶绿素荧光信号中分离出荧光衰减函数，可获取荧光寿命估计值。再结合叶绿素荧光寿命校正技术就能反演得到高精度的植物荧光寿命。仿真与实验结果表明：该方法可实现高精度的植物荧光寿命实时监测；并对不同含量的叶绿素提取液进行了测试，构建了植物荧光寿命与叶绿素含量的对应关系模型。未来该技术可用于遥感监测海洋、湖泊、河流中藻类植物的生物含量。

Laser-Induced Plant Fluorescence Lifetime Correction Technique and Its Properties Analysis

Laser-induced chlorophyll fluorescence lifetime measurement is a novel technique for evaluating plant growth status and environmental monitoring. This method is developed on the basis of plant fluorescence spectrum analysis technique. According to the physical properties of chlorophyll fluorescence signal and information simulation technology, a laser-induced chlorophyll fluorescence lifetime correction technique for improving the chlorophyll fluorescence lifetime measurement accuracy is presented in this paper. Laser-induced plant fluorescence lifetime measurement system was used to collect the chlorophyll fluorescence and its background signal. Then, the chlorophyll fluorescence decay function can be separated from the plant fluorescence signal in deconvolution algorithm, and fluorescence lifetime estimation can be obtained. Finally, the chlorophyll fluorescence lifetime correction technique was used to retrieve the precision values of fluorescence lifetime. The results of simulation and experiment show that real-time monitoring of high-precision chlorophyll fluorescence lifetime can be achieved in this method. Based on this, a large number of different concentrations chlorophyll solution were measured, and the relationship model of chlorophyll content and the fluorescence lifetime was built. In the future, this method can be used for remote sensing to monitor the algae biomass in the ocean, lake or river.