农垦58s育性转换期间叶片核蛋白因子与LRE结合研究

运用人工合成的ＬＲＥ序列，采用Ｂａｎｄｓｈｉｆｔａｓｓａｙ方法分析了农垦５８ｓ和农垦５８品种叶片中核蛋白因子与ＬＲＥ的结合反应．研究结果表明：在农垦５８Ｓ的育性转换敏感期叶片中存在与ＬＲＥ结合的核蛋白因子，这种核蛋白因子受磷酸化的调节；认为在长日照下农垦５８ｓ叶片中的核蛋白因子以磷酸化的形式与ＬＲＥ结合，磷酸化促进了核蛋白二聚化过程．同时比较了农垦５８ｓ和农垦５８品种叶片中核蛋白因子与ＬＲＥ结合反应的差别，二者的叶片中的核蛋白不仅与ＬＲＥ的结合活性存在差异，而且其调节方式明显不同．

THE STUDY ON THE SPECIFIC INTERACTION BETWEEN LRE AND NUCLEAR PROPEINS OF LEAVES IN PGMR DURING FERTILITY TRANSFORMATION

We analysed the binding intereaction of nuclear proteins of leaves in Nongken 58 s and Nongken 58 variety by Bandshift assay, using a 37 hp synthetic LRE from rbcs genes. The results indicated that the nuclear proteins specifically binding to LRE existed in the two varieties in leaves during fertility transformation. The binding activity is stimulated by phosphorylation of the nuclear proteins,meanwhile,the stimulation accompanied with disappearing and weakening of another binding band. It suggested that the nuclear proteins in leaves of Nongken 58 s Under longday may be dimer of the nuclear proteins specifically binding to LRE, which are regulated by phosphorylatin. The comparison of interaction of the nuclear proteins with LRE between Nongken 58 s and Nongken 58 variety indicated that they were not only different from the binding bands, but also different from the regulation pattern.