三苯基锡化合物与牛血清白蛋白作用光谱

三苯基锡化合物与牛血清白蛋白作用光谱;三苯基一氯化锡(TPTCl); 牛血清白蛋白(BSA); 光谱

Interaction of Chlorotriphenyltin with BSA

Chlorotriphenyltin(TPTCl) is one of the major components of ship fouling paints. The spectral (characteristics) of the interaction of TPTCl with Bovine Serum Albumin(BSA) were studied. The results show that BSA coordinates with the tin ion of the TPTCl, resulting in the exposure of aromatic heterocycle residues of tryptophane and tyrosine in BSA moledule, the (benzene) ring of TPTCl interacts with BSA through hydrophobic action, which leads to contraction of BSA molecules. The (obvious) hyperchromic effect was observed in the UV spectra at the beginning, which then weakened with the reaction time. In addition, the UV spectra and the CD spectra show that TPTCl (mainly) interacts with BSA through coordination when the TPTCl concentration is low, whereas the hydrophobic interaction dominates when the TPTCl concentration is high. The various buffer solutions added favors the interaction of TPTCl and BSA to different extent. The order(from strong to weak) of hyperchromic effects was KH_(2)PO_(4)-NaOH system, Tris-HCl system, HAc-NaAc system, and no buffer system.