Dynamics of cellulase production by glucose grown cultures of Trichoderma reesei Rut-C30 as a response to addition of cellulose |
| |
Authors: | Nóra Szijártó Zsolt Szengyel Gunnar Lidén Kati Réczey |
| |
Institution: | (1) Department of Agricultural Chemical Technology, Budapest University of Technology and Economics, Szent Gellért tér 4, H-1521 Budapest, Hungary;(2) Department of Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden |
| |
Abstract: | An economic process for the enzymatic hydrolysis of cellulose would allow utilization of cellulosic biomass for the production
of easily fermentable low-cost sugars. New and more efficient fermentation processes are emerging to convert this biologic
currency to a variety of commodity products with a special emphasis on fuel ethanol production. Since the cost of cellulase
production currently accounts for a large fraction of the estimated total production costs of bioethanol, a significantly
less expensive process for cellulase enzyme production is needed. It will most likely be desirable to obtain cellulase production
on different carbon sources—including both polymeric carbohydrates and monosaccharides. The relation between enzyme production
and growth profile of the microorganism is key for designing such processes. We conducted a careful characterization of growth
and cellulase production by the soft-rot fungus Trichoderma reesei. Glucosegrown cultures of T. reesei Rut-C30 were subjected to pulse additions of Solka-floc (delignified pine pulp), and the response was monitored in terms
of CO2 evolution and increased enzyme activity. There was an immediate and unexpectedly strong CO2 evolution at the point of Solka-floc addition. The time profiles of induction of cellulase activity, cellulose degradation,
and CO2 evolution are analyzed and discussed herein. |
| |
Keywords: | Trichoderma reesei fermentation cellulase growth characterization cellulose hydrolysis |
本文献已被 SpringerLink 等数据库收录! |
|