Simultaneous Determination of Rosiglitazone and Metformin in Pharmaceutical Preparations by LC |
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Authors: | Ceren Yard?mc? Nuran Özalt?n |
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Institution: | (1) Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, 06100 Sıhhıye, Ankara, Turkey |
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Abstract: | In the present study, a novel, fast and simple liquid chromatographic method was developed and validated for the simultaneous
determination of rosiglitazone and metformin in pharmaceutical preparations. The separation was achieved on a phenyl column
(250 × 4.6 mm i.d., 5 μm) using a mobile phase composed of acetonitrile:10.0 mM phosphate buffer pH 5.5 (70:30, v/v). The flow rate was 1 mL min−1. UV detection was performed at 245 nm and verapamil was used as internal standard. The developed method was validated in
terms of stability, specificity, sensitivity, linearity, accuracy, precision and robustness. The limit of quantification was
0.02 μg mL−1 for both drugs. The method developed was successfully applied to the simultaneous determination of rosiglitazone and metformin
in pharmaceutical preparations. The results were compared to two methods reported in the literature and no significant difference
was found statistically. |
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Keywords: | Column liquid chromatography Rosiglitazone and metformin Validation Drug analysis |
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