| PFOS对哈维氏弧菌生长及外毒素基因的影响 |
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| 引用本文： | 舒波,黄德贵,关万春,潘长旺,常彦祥.PFOS对哈维氏弧菌生长及外毒素基因的影响[J].广东微量元素科学,2011,18(9):44-49. |
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| 作者姓名： | 舒波 黄德贵 关万春 潘长旺 常彦祥 |
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| 作者单位： | 1. 湖南永清环保股份有限公司,湖南 长沙,410005
2. 温州医学院,浙江 温州,325035 |
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| 基金项目： | 温州科技计划项目(Y20080001) |
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| 摘 要： | 探讨了全氟辛烷磺酸盐(PROS)对哈维氏弧菌密度及外毒素基因表达的影响.用含有不同浓度PFOS(5、50、500 mmol/L)的培养基培养哈维氏弧菌,分别在6、15、24 h取适量菌液,经4 000 r/min离心,磷酸盐缓冲液(PBS)洗涤3次,制成PBS菌悬液.采用实时荧光定量PCR检测其胞外蛋白酶、溶血素及保守...
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| 关 键 词： | 全氟辛烷磺酸盐 哈维氏弧菌 胞外蛋白酶 溶血素 实时荧光定量PCR |
Impact of PFOS on Growth and ECP Gene of Vibrio harveyi |
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| SHU Bo,HUANG Degui,GUAN Wanchun,PAN Changwang,CHANG Yanxiang.Impact of PFOS on Growth and ECP Gene of Vibrio harveyi[J].Trace Elements Science,2011,18(9):44-49. |
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| Authors: | SHU Bo HUANG Degui GUAN Wanchun PAN Changwang CHANG Yanxiang |
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| Institution: | SHU Bo2,HUANG Degui1,GUAN Wanchun1,PAN Changwang1,CHANG Yanxiang1(1.Wenzhou Medical College,Wenzhou 325035,China,2.Hunan Yongqing Envirojnmental Conservation Ltd-Co,Changsha 410005,China) |
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| Abstract: | The effect of perfluorooctane sulphonate(PFOS) on the Vibrio harveyi cell density and expression of extra-toxin gene was investigated.The Vibrio harveyi samples,which cultured in LB media with different PFOS concentrations(5,50,500 mmol/L),were collected by centrifugation(4 000 r/min) after 6,15 and 24 h incubation,respectively,and cell sediments were re-suspend in phosphate buffer solution(PBS).And the RT-PCR was used for analyzing the gene expression of extracellular protease,hemolysin and 16 SrRNA conser... |
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| Keywords: | PFOS Vibrio harveyi extracellular protease hemolysin real-time PCR |
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