| 免疫共振散射分析法测定人血清中痕量脂蛋白(a) |
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| 引用本文： | 孙双姣,刘梯楼. 免疫共振散射分析法测定人血清中痕量脂蛋白(a)[J]. 广东微量元素科学, 2011, 18(9): 50-53 |
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| 作者姓名： | 孙双姣 刘梯楼 |
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| 作者单位： | 邵阳医学高等专科学校,湖南 邵阳,422000 |
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| 基金项目： | 邵阳市科技局科技项目(Z0901) |
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| 摘 要： | 在pH为7.6的Tris - HCI缓冲溶液及聚乙二醇6000 (PEG 6000)存在下,羊抗人脂蛋白(a)与脂蛋白(a)发生免疫反应形成疏水性免疫复合物微粒,导致体系的共振散射强度增强,在波长为340 nm处具有较强的共振散射峰.在选定条件下,脂蛋白(a)质量浓度在0.10～2.33μg/mL范围内与340 nm处...
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| 关 键 词： | 羊抗人脂蛋白(a) 脂蛋白(a) 免疫复合物 免疫共振散射 |
Immune Resonance Scattering Spectral Method for Determination of Lipoprotein(a) in Plasma |
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| SUN Shuangjiao,LIU Titou. Immune Resonance Scattering Spectral Method for Determination of Lipoprotein(a) in Plasma[J]. Trace Elements Science, 2011, 18(9): 50-53 |
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| Authors: | SUN Shuangjiao LIU Titou |
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| Affiliation: | SUN Shuangjiao,LIU Titou(Shaoyang Medical College,Shaoyang 422000,China) |
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| Abstract: | In pH 7.6 Tris-HCl buffer solution,goat anti-human lipoprotein（a） combine with lipoprotein（a） to form immune complex particles that exhibit a stronger resonance scattering at 340 nm.The lipoprotein（a） concentration ρ in the range of 0.10～2.33 μg/mL is linear to the resonance scattering intensity 340 nm.The regress equation is ΔIRS=110 ρ-26.667,the coefficient is 0.996.Its detection limit is achieve at 0.067 μg/mL lipoprotein（a）.The proposed method was applied to the determination of lipoprotein（a） in plasma,with simplicity,sensitivity,good selectivity and satisfactory results. |
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| Keywords: | goat anti-human lipoprotein(a) lipoprotein(a) immune complex particles resonance scattering spectral method |
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