A fast atom bombardment and matrix-assisted laser desorption/ionization mass spectrometry study of doubly charged porphyrins |
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Authors: | Schäfer M Budzikiewicz H |
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Institution: | Mass Spectrometry Facilities, Institute of Organic Chemistry, University at Cologne Greinstr. 4, D-50939 Cologne, Germany. mathias.schaefer@uni-koeln.de |
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Abstract: | In this mass spectrometry (MS) study of doubly charged porphyrin salts, fast atom bombardment (FAB) and matrix-assisted laser desorption/ionization (MALDI) MS techniques are utilized to examine several unique ionic species. The predominant transformation of preformed doubly charged ions in the desorption/ionization mechanism of FAB and MALDI is the result of deprotonation reactions to form singly charged ions of the type M(2+) - H(+)](+) and of one-electron reductions to form radical cations M(2+) + e(-)](+.). The dependence of this phenomenon and the formation of a number of additional ionic species on the different matrices and the FAB-matrix additive benzoquinone is examined. The significant analogous behavior of doubly charged porphyrins in FAB- and MALDI-MS leads to the conclusion that one-electron reductions are of distinct relevance in the desorption/ionization mechanism of MALDI. |
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