Colorimetric detection of hepatitis B virus (HBV) DNA based on DNA-templated copper nanoclusters |
| |
Authors: | Xiaoxia Mao Siyu Liu Chao Yang Fengzhen Liu Keming Wang Guifang Chen |
| |
Institution: | 1. Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China;2. Department of Life Science, Anqing Normal University, Anqing, Anhui 246011, China;3. Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China |
| |
Abstract: | DNA detection plays an important role in early diagnosis of genetic disease. The conventional detection methods of DNA are based on expensive equipment, which do not meet the demands of developing countries. Thus, we developed a colorimetric method, which could be observed with naked eye and used copper nanoclusters for cost-effective. Moreover, the target of this method is the DNA in Hepatitis B virus that is one of the most popular chronic viral infections in developing countries over the past years. Our method was sensitive and the limit of detection was 12 × 109 molecules. Three-base-pair mismatches target DNA was detected easily. These results revealed the favorable sensitivity and selectivity of this approach. Most importantly, our method may have potential applications in correct diagnosis of genetic disease and monitoring of gene therapy in the poverty-stricken areas. |
| |
Keywords: | DNA Hepatitis B virus Colorimetry Copper nanoclusters Catalysis Biosensor |
本文献已被 ScienceDirect 等数据库收录! |
|