A novel automated device for rapid nucleic acid extraction utilizing a zigzag motion of magnetic silica beads |
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Authors: | Akemi Yamaguchi Kazuyuki Matsuda Masayuki Uehara Takayuki Honda Yasunori Saito |
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Affiliation: | 1. Graduate School of Science and Technology, Shinshu University, Nagano, Japan;2. Core Technology Development Center, Seiko Epson Corporation, Suwa, Japan;3. Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan;4. Institute of Engineering, Academic Assembly, Shinshu University, Nagano, Japan |
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Abstract: | We report a novel automated device for nucleic acid extraction, which consists of a mechanical control system and a disposable cassette. The cassette is composed of a bottle, a capillary tube, and a chamber. After sample injection in the bottle, the sample is lysed, and nucleic acids are adsorbed on the surface of magnetic silica beads. These magnetic beads are transported and are vibrated through the washing reagents in the capillary tube under the control of the mechanical control system, and thus, the nucleic acid is purified without centrifugation. The purified nucleic acid is automatically extracted in 3 min for the polymerase chain reaction (PCR). The nucleic acid extraction is dependent on the transport speed and the vibration frequency of the magnetic beads, and optimizing these two parameters provided better PCR efficiency than the conventional manual procedure. There was no difference between the detection limits of our novel device and that of the conventional manual procedure. |
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Keywords: | Nucleic acid extraction Magnetic silica beads Zigzag motion Automated device |
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