Suicide inhibition of alpha-oxamine synthases: structures of the covalent adducts of 8-amino-7-oxononanoate synthase with trifluoroalanine |
| |
Authors: | Alexeev Dmitriy Baxter Robert L Campopiano Dominic J Kerbarh Olivier Sawyer Lindsay Tomczyk Nicholas Watt Rory Webster Scott P |
| |
Institution: | School of Biology, Swann Building, University of Edinburgh, Mayfield Road, Edinburgh, UKEH9 3JR. |
| |
Abstract: | The irreversible inhibition of 8-amino-7-oxononanoate synthase by trifluoroalanine involves decarboxylative defluorination of the inhibitor-PLP aldimine followed by attack of the conjugated imine by the amino group of the active site lysine to afford a covalently bound difluorinated intermediate which can subsequently undergo further HF losses and hydrolysis to afford a 2-(pyridoximine phosphate) acetoyl protein adduct. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|