| HPLC-DAD测定利培酮中有关物质 |
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| 引用本文： | 曹天海. HPLC-DAD测定利培酮中有关物质[J]. 光谱实验室, 2010, 27(4): 1473-1476 |
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| 作者姓名： | 曹天海 |
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| 作者单位： | 昆明积大制药有限公司,昆明市高新区科新路389号,650106 |
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| 摘 要： | 运用二极管阵列检测器结合峰纯度分析建立利培酮有关物质HPLC的测定方法。采用C18色谱柱(Kromasil,C18,5μm,4.6×200mm),以1.0%三乙胺缓冲溶液(以磷酸调节pH值至3.0)∶乙腈(70∶30)为流动相,流速为0.8mL/min,柱温为30℃,检测波长为260nm。利培酮与有关物质分离良好,原料1、中间体1、中间体2以及利培酮检出限分别为0.4、4、0.4、0.6ng。二极管阵列检测器结合峰纯度分析可用于HPLC测定利培酮有关物质时色谱条件的选择和评价,本方法简单、快速、专属性好、灵敏度高。
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| 关 键 词： | 高效液相色谱法 二极管阵列检测器 峰纯度分析 利培酮 有关物质 |
Determination of Related Substances in Risperidone by HPLC-DAD |
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| CAO Tian-Hai. Determination of Related Substances in Risperidone by HPLC-DAD[J]. Chinese Journal of Spectroscopy Laboratory, 2010, 27(4): 1473-1476 |
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| Authors: | CAO Tian-Hai |
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| Affiliation: | CAO Tian-Hai(Kunming Jida Pharmaceutical Co.,Ltd.,Kunming 650106,P.R.China) |
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| Abstract: | A new HPLC method to determine related substances in risperidone by DAD and peak purity analysis was established,using C18 column（Kromasil,C18,5μm,4.6×200mm）,acetonitrile-1% triethylamine buffer（30：70）（adjust pH to 3.0 by phosphoric acid）as mobile phase.The flow rate is 0.8mL/min;the column oven temperature is 30℃;the detection wavelength is 260nm.Risperidone and related substances are well separated; the LOD of raw material 1,intermediate 1,and intermediate 2 were 0.4,4,0.4,and 0.6ng.DAD and peak purity analysis is useful for evaluation of determination of related substances in risperidone by HPLC; the method is simple,fast,specific and sensitive. |
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| Keywords: | HPLC DAD Peak Purity Analysis Risperidone Related Substance |
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