| 流动注射碱性降解荧光法快速测定微量金霉素 |
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| 引用本文： | 赵一兵 余志雄. 流动注射碱性降解荧光法快速测定微量金霉素[J]. 高等学校化学学报, 1992, 13(10): 1233-1236 |
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| 作者姓名： | 赵一兵 余志雄 |
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| 作者单位： | 厦门大学化学系，厦门大学化学系，厦门大学化学系，厦门大学化学系，厦门大学化学系 厦门361005，厦门361005 1991届本科毕业生，厦门361005，厦门361005，厦门361005 |
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| 摘 要： | 提出了流动注射碱性降解荧光法快速测定微量金霉素的新方法.金霉素在流动管路中与0.25 mol/L NaOH溶液快速反应,生成强荧光降解物.在流通池中用荧光法进行检测.反应产物荧光强度在10~(-8)～10~(-5)mol/L范围与金霉素含量成正比.共存四环素和脱水金霉素杂质不干扰金霉素测定.本法既可用于金霉素产品含量测定,又可用于四环素产品中杂质金霉素的测定,还成功地应用于尿液和血清中微量金霉素的测定.
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| 关 键 词： | 流动注射分析 荧光法 金霉素 |
Rapid Alkaline Degradation Determination of Trace Chlorotetracycline by Flow Injection Fluorimetry |
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| ZHAO Yi-Bing,YU Zhi-Xiong,GUO Xiang-Qun,XU Jin-Gou,CHEN Guo-Zhen. Rapid Alkaline Degradation Determination of Trace Chlorotetracycline by Flow Injection Fluorimetry[J]. Chemical Research In Chinese Universities, 1992, 13(10): 1233-1236 |
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| Authors: | ZHAO Yi-Bing YU Zhi-Xiong GUO Xiang-Qun XU Jin-Gou CHEN Guo-Zhen |
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| Abstract: | The present paper covers a new flow injection fluorimetric method for the determination of trace chlorotetracycline.Chlorotetracycline reacts rapidly with 0.25 mol/L NaOH in a reaction tube and produces strong fluorescent species detected by fluorimetry in a flow cell.The fluorescence intensity of the product is proportional to the concentration of chlorotetracycline over the range of 10-8~10-5 mol/L.Coexisting tetracycline and anhydrochlorotetracycline do not interfere with the determination of chlorotetracycline.This method can be used not only for the quality detection of chlorotetracycline medicine but also for the determination of chlorotetracycline in tetracyline medicine.The determination of chlorotetracyciine in urine and serum is successful with satisfactory recoveries. |
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| Keywords: | Flow injection analysis Alkaline degradation Fluorimetry Chlorotetracycline |
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