A Highly Selective and Sensitive Ratiometric Fluorescent Probe for pH Measurement Based on Fluorescence Resonance Energy Transfer |
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Authors: | LIANG Fanghui WANG Di MA Pinyi WANG Xinghua SONG Daqian YU Yong |
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Institution: | 1. Department of Pharmacy, Changchun Medical College, Changchun 130031, P. R. China;
2. College of Chemistry, Jilin University, Changchun 130012, P. R. China;
3. College of Instrumentation & Electrical Engineering, Jilin University, Changchun 130061, P. R. China |
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Abstract: | Fluorescein-rhodamine 6G(Flu-Rh) was synthesized and used as the fluorescence probe for pH measurement based on fluorescence resonance energy transfer(FRET). In the probe, fluorescein fluorophore and pH-sensitive rhodamine 6G hydrazide were used as FRET donor and acceptor, respectively. The values of acidity constant(pKa) and fluorescence quantum yield(Φ) of Flu-Rh were 3.71 and 0.72, respectively. The fluorescence efficiency of Flu-Rh remains almost constant when the pH value of the sample solution changed 10 times in a range of 4.78-7.03 continuously. The present probe is simple and easy-to-use for the pH measurement in acidic media. |
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Keywords: | Fluorescence resonance energy transfer(FRET) Ratiometric Fluorescein Rhodamine 6G pH |
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