| AgNO3和低温处理对小麦细胞GST及GR酶活性的影响 |
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| 引用本文： | 杜建芳,廖祥儒,侯小康,王俊丽,陈丕铃,周艳芬,芦春斌,王建平.AgNO3和低温处理对小麦细胞GST及GR酶活性的影响[J].河北大学学报(自然科学版),2001,21(4):402. |
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| 作者姓名： | 杜建芳 廖祥儒 侯小康 王俊丽 陈丕铃 周艳芬 芦春斌 王建平 |
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| 作者单位： | 1.河北大学,生命科学学院,河北,保定,071002； 2.暨南大学,生殖免疫中心,广东,广州,510632 |
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| 基金项目： | 国家 8 6 3计划项目，河北省教委博士基金资助项目，河北大学基金资助项目 |
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| 摘 要： | 研究了AgNO3和低温处理对小麦悬浮细胞谷胱甘肽转移酶(GST)及谷胱甘肽还原酶(GR)酶活性的影响.结果表明,AgNO3和低温处理对小麦细胞再生的影响因处理时间的不同而有差异,在处理的4 d内低温处理使再生率明显增加,AgNO3处理2 d使细胞再生率显著增高,但处理4 d则对细胞再生无明显影响.低温处理使细胞蛋白质含量明显升高,AgNO3处理对小麦细胞蛋白质含量无明显影响.小麦细胞GST活性随处理时间的延长而迅速降低,AgNO3处理对GST活性无明显影响,但低温处理明显延缓GST活性降低.AgNO3和低温处理都使小麦细胞GR酶活性明显降低.AgNO3处理2 d和低温处理对细胞谷胱甘肽(GSH)含量无明显影响,但在处理的第4 d AgNO3使细胞GSH含量明显降低.
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| 关 键 词： | GST GSH 小麦 悬浮细胞 低温 AgNO3 |
Effect of AgNO3 and Low Temperature on the Activity of Glutathione S-Transferase and Glutathione Reductase in Cells of Wheat |
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| DU Jian_fang ,LIAO Xiang_ru ,HOU Xiao_kang ,WANG Jun_li ,CHEN Pi_ling ,ZHOU Yan_fen ,LU Chun_bin ,WANG Jian_ping.Effect of AgNO3 and Low Temperature on the Activity of Glutathione S-Transferase and Glutathione Reductase in Cells of Wheat[J].Journal of Hebei University (Natural Science Edition),2001,21(4):402. |
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| Authors: | DU Jian_fang LIAO Xiang_ru HOU Xiao_kang WANG Jun_li CHEN Pi_ling ZHOU Yan_fen LU Chun_bin WANG Jian_ping |
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| Institution: | DU Jian_fang 1,LIAO Xiang_ru 1,HOU Xiao_kang 1,WANG Jun_li 1,CHEN Pi_ling 1,ZHOU Yan_fen 1,LU Chun_bin 2,WANG Jian_ping 1 |
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| Abstract: | Effects of AgNO 3 and chilling on cell regeneration and activity of glutathione S-transferase(GST) and glutathione reductase (GR) in suspension cells of wheat were investigated. The data showed that influence of both AgNO 3 and chilling in cell regeneration changed with the length of treating time, cell regeneration was obviously improved by AgNO 3 in 2 days and chilling in 4 days. The content of protein was increased by chlling, but not influenced by AgNO 3. The activity of GST decreased with the treating time, these procedure was retarded by chilling but not by AgNO 3. Both chilling and AgNO 3 reduced the activity of GR. The content of GSH was not influenced with AgNO 3 for 2 days and with chilling, but reduced significantly by AgNO 3 for 4 days. |
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| Keywords: | GST GSH wheat suspension cell chilling AgNO 3 |
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