Pulsed field electrophoresis in contour-clamped homogeneous electric fields for the resolution of DNA by size or topology |
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Authors: | G Chu |
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Institution: | Department of Medicine, Stanford University, CA 94305. |
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Abstract: | The electrophoretic separation of DNA molecules can be controlled by the use of contour-clamped homogeneous electric fields (CHEF). This paper describes an improved CHEF apparatus with negligible distortion in the electric fields. When the electric field was periodically reoriented, DNA molecules up to 2 megabases were resolved in a highly uniform manner. Furthermore, when the field strength was changed with orientation, topological variations of conventional-sized DNA molecules were resolved. |
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