Multiplex-polymerase chain reaction assay for the authentication of the mackerel Scomber colias in commercial canned products |
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| Authors: | Infante Carlos Manchado Manuel |
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| Institution: | Laboratorio de Identificación de Especies Pesqueras y Acuicolas, CIFPA El Toru?o, I.F.A.P.A. C.I.C.E. (Junta de Andalucia), 11500 El Puerto de Santa Maria Cádiz, Spain. carlos.infante.ext@juntadeandalucia.es |
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| Abstract: | A multiplex-polymerase chain reaction (PCR) system was developed for the authentication of the mackerel Scomber colias in commercial canned products. This novel method consists of an S. colias-specific fragment 159 base pairs (bp)] located in the nontranscribed spacer (NTS) sequence, and a Scomber genus-specific PCR product in the 5S rRNA gene (196-201 bp) as a positive amplification control. The system was assayed using 18 different canned products labeled as S. colias. A positive identification was made in all but one sample, revealing this methodology as a potential molecular tool for direct application in the authentication of S. colias canned products. |
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