Single-shot morpho-functional and structural characterization of the left-ventricle in a mouse model of acute ischemia-reperfusion injury with an optimized 3D IntraGate cine FLASH sequence at 7T MR |
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| Institution: | 1. Yuhuangding Hospital, Qingdao University School of Medicine, Yantai, China;2. Department of Radiology, University of Chicago, Chicago, Illinois;3. Department of Radiology, The Affiliated Hospital of Qingdao University, Shandong, China;1. Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland;2. Department of Cardiology, Medical University of Lodz, Poland;3. Traumatology and Orthopaedics Department, District Hospital of Wielkopolska, Poznan, Poland;1. Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan;2. Department of Medical Biochemistry, Osaka University Graduate School of Frontier Bioscience, Suita, Osaka 565-0871, Japan;3. Department of Biomedical Imaging, National Cardiovascular and Cerebral Research Center, Suita, Osaka 565-8565, Japan;4. Comprehensive Heart Failure Center, University of Wuerzburg, Wuerzburg 97078, Germany;5. Department of Nuclear Medicine, University of Wuerzburg, Wuerzburg 97078, Germany;6. Department of Medical Physics and Engineering, Division of Health Sciences, Osaka University Graduate School of Medicine, Suita, Osaka 560-0871, Japan |
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| Abstract: | Preclinical cardiac MR is challenging and time-consuming. A fast and comprehensive acquisition protocol and standardized image post-processing may improve preclinical research, reducing acquisition time, costs and variability of results. In the present study, we evaluated the feasibility of a contrast-enhanced 3D IntraGate steady-state cine sequence (ce-3D-IG-cine) with short acquisition time (11 min) for a single-shot combined characterization of left ventricle (LV) remodeling and infarct size (IS) in a mouse model of acute ischemia-reperfusion injury.Sixteen male C57BL/6N mice underwent 7T cardiac MR (Bruker, BioSpec 70/30) including optimized ce-3D-IG-cine (total scan time 11 min) at day 1, 5 and 28 after surgery. LV end-diastolic volume (EDVMR) and ejection fraction (EFMR) extracted from MR were compared to ones from short-axis (SA-EDVecho, SA-EFecho) and parasternal long-axis (LA-EDVecho, LA-EFecho) echocardiography. IS was manually and semiautomatically segmented from ce-3D-IG-cine using different standard deviation (SD +2, +3, +4, +5, +6 in respect to a reference tissue).Mice were sacrificed at day 28, immediately after imaging. IS at day 28 was compared to injury burden at histology.MR and echocardiographic morpho-functional parameters were compared, as IS from MR and histology. Bland-Altman plots were used to assess the agreement in ischemic burden segmentation.Volumetric and functional parameters measured on ce-3D-IG-cine correlated to the correspondent echocardiographic parameter (EDVMR vs SA-EDVecho: ρ = 0.813; EDVMR vs LA-EDVecho: ρ = 0.845; EFMR vs SA-EFecho ρ = 0.612; EFMR vs LA-EFecho ρ = 0.791; p < 0.001 in all cases).Manually segmented IS strongly correlated with the scar at histology (ρ = 0.904, p < 0.001). A threshold of +3SD showed the highest performance for semiautomatic assessment of IS compared to manual segmentation (ρ = 0.965, p < 0.001), with an overall reproducibility of 73%, and a peak reproducibility of 80% at day 1.The ce-3D-IG-cine sequence, manually or semiautomatically segmented using 3SD threshold, allows fast and comprehensive LV morpho-functional and structural characterization in myocardial ischemia-reperfusion injury model. |
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