| 茶叶样品中绿原酸含量的高效液相色谱法测定 |
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| 引用本文： | 王丽,帅琴,汤志勇,金泽祥. 茶叶样品中绿原酸含量的高效液相色谱法测定[J]. 分析测试学报, 2001, 20(1): 76-78 |
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| 作者姓名： | 王丽 帅琴 汤志勇 金泽祥 |
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| 作者单位： | 中国地质大学材料科学与化学工程学院, |
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| 摘 要： | 建立了茶叶绿原酸含量测定的择相高效液相色谱法，采用PecosphereC18柱，以甲醇－水（体积比18：20，水中含φ＝1／61的甲酸）淋洗，用紫外检测器于326nm处测定，各组分的色谱峰达到基线分离。绿原酸在0．02－4．02μg范围内线性关系良好，线性相关系数 r＝0．9996。在安徽黄山产贡菊和安徽产金银花中加标的回收率分别为86．8％、102．0％，该法已用于十多种茶趺实际样品的测定。
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| 关 键 词： | 茶叶 绿原酸 高效液相色谱法 含量测定 |
| 文章编号： | 1004－4957（2001）01－0076－03 |
| 修稿时间： | 2000-04-16 |
Determination of Chlorogenic Acid in Tea by High Performance Liquid Chromatography |
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| WANG Li. Determination of Chlorogenic Acid in Tea by High Performance Liquid Chromatography[J]. Journal of Instrumental Analysis, 2001, 20(1): 76-78 |
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| Authors: | WANG Li |
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| Abstract: | A sensitive and precise method has been developed for thedetection and quantitation of chlorogenic acid in tea. The chlorogenic acid was seperated from methanol extract of tea by RP-HPLC with pecosphere C18 column and detected with UV detector at 326 nm. The mobile phase consisted of solvent A(methanol) and solvent B(water including 1/61(φ) formic acid). The column was eluted with following gradient: 0～17 min 18％ A; 18～27 min 100％ A. The calibration curve is linear in the range of 0.02～4.02 μ g for chlorogenic acid. The spiked recovery rates are 86.8％ and 102.0％ for Gongju tea in Huang Shan and Honeysuckle tea in Anhui, respectively. The method has been applied to practical samples over 10 kinds of tea. |
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| Keywords: | HPLC Chlorogenic acid Tea |
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