Improved method for the solid-phase synthesis of oligoribonucleotide 5'-triphosphates |
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Authors: | Seigo Nagata Kazuchika Takagaki Takeshi Wada |
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Affiliation: | Discovery Research Laboratories, Nippon Shinyaku Co., Ltd. |
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Abstract: | We have developed an improved solid-phase method for the synthesis of 5'-triphosphates (5'-TPs) of oligoribonucleotides. The method is based on the use of salicyl phosphorochloridite as the phosphitylating reagent and the improvement is characterized by the use of the highly reactive pyrophosphorylating reagent tris(tetra-n-butylammonium) hydrogen pyrophosphate instead of the conventional tri-n-butylammonium salt for the nucleophilic substitution reaction to form the cyclic ester intermediate. The improved method can be used to generate oligoribonucleotide 5'-TPs efficiently and reproducibly. |
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