| 高效液相色谱法测定平胃丸中橙皮苷的含量 |
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| 引用本文： | 轩凤国,田宝勇,董立龙,杜秀芳,王伟,高媛.高效液相色谱法测定平胃丸中橙皮苷的含量[J].理化检验(化学分册),2012(1):93-94,103. |
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| 作者姓名： | 轩凤国 田宝勇 董立龙 杜秀芳 王伟 高媛 |
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| 作者单位： | 北京同仁堂天然药物(唐山)有限公司;河北科技大学;河北医科大学药学院 |
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| 基金项目： | 河北省卫生厅中医、中西医结合科研计划(No.05057) |
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| 摘 要： | 提出了高效液相色谱法测定平胃丸中橙皮苷含量的方法。采用Hypersil BDS C18(200mm×4.6mm,5μm)色谱柱;以甲醇-乙酸-水(35+4+61)混合溶液为流动相,流量1mL.min-1,检测波长为283nm,橙皮苷的质量浓度与峰面积在8.08～121.2mg.L-1范围内呈线性关系。方法用于平胃丸中橙皮苷的测定,平均回收率为101.1%,相对标准偏差(n=9)为1.2%。
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| 关 键 词： | 高效液相色谱法 平胃丸 橙皮苷 |
HPLC Determination of Hesperidin in the TCM "Pingwei Wan" |
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| XUAN Feng-guo,TIAN Bao-yong,DONG Li-long,DU Xiu-fang,WANG Wei,GAO Yuan.HPLC Determination of Hesperidin in the TCM "Pingwei Wan"[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2012(1):93-94,103. |
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| Authors: | XUAN Feng-guo TIAN Bao-yong DONG Li-long DU Xiu-fang WANG Wei GAO Yuan |
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| Institution: | 1.Beijing Tongrentang Naturepharm(Tangshan) Co.,Ltd.,Tangshan 064100,China; 2.Hebei University of Science and Technology,Shijiazhuang 050018,China; 3.Department of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China) |
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| Abstract: | HPLC was applied to the determination of hesperidin in the prepared tradition Chinese medicine named "Pingwei Wan".The Hypersil BDS C18 column(250 mm×4.6 mm,5 μm) was used as stationary phase,and a mixture of methanol,acetic and water,mixed in the ratio of 35 to 4 to 61 by volume,was used as mobile phase with a flow-rate of 1 mL·min-1.Detection at the wavelength of 283 nm was adopted in the determination.Linear relationship between values of peak area and mass concentration of hesperidin was obtained in the range of 8.08-121.2 mg·L-1.The proposed method was used in the determination of hesperidin in the drug,giving average recovery of 101.1% with RSD(n=9) 1.2%. |
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| Keywords: | HPLC Pingwei Wan Hesperidin |
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