Study of two-dimensional liquid chromatography with high temperature NPLC and room temperature RPLC |
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Authors: | Hongzhe Tian Jing Xu Kun Ding Yafeng Guan |
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Affiliation: | (1) Department of Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China;(2) Analytical Center, Shenyang Agricultural University, Shenyang, 110161, China |
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Abstract: | To overcome the peak band broadening and to increase the peak capacity and separation efficiency of a two-dimensional liquid chromatographic system, a high-temperature normal phase liquid chromatography (HTNPLC) was used as the first dimension (1st-D), and a RPLC was used as the second dimension (2nd-D). The sample was first separated on the 1st-D CN column and the primary eluent stored in the sampling-loop system alternatively (in HTNPLC×RPLC mode) or selectively (in HTNPLC/RPLC mode) and was then transferred to 2nd-D C18 column for further separation. The resolution and separation efficiency of the systems were greatly improved. The systems were evaluated by analyzing several polycyclic aromatic hydrocarbons and Glycyrrhiza uralensis extract. __________ Translated from Journal of Instrumental Analysis, 2008, 27(1) (in Chinese) |
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Keywords: | two-dimensional liquid chromatography HTNPLC /RPLC sample loops- valve interface traditional Chinese medicine Glycyrrhiza uralensis |
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