Dioxin analysis in feed: cell-based assay versus mass spectrometry method |
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Authors: | Marie-Louise Scippo Soledad Rybertt Gauthier Eppe Anne-Cécile Massart Edwin De Pauw Guy Maghuin-Rogister |
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Institution: | (1) CART Laboratory of Analysis of Foodstuffs of Animal Origin, University of Liège, Boulevard de Colonster 43bis Sart-Tilman, 4000, Liège, Belgium;(2) CART Mass Spectrometry Laboratory, Chemistry Department, University of Liège, Allée de la Chimie 3, B-6c Sart-Tilman, 4000, Liège, Belgium |
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Abstract: | In the determination of contaminants (dioxins, polychlorinated biphenyls, polyaromatic hydrocarbons), cell-based assays are
useful methods for screening purposes: they are mainly characterized by high sample throughput and lower costs than the Mass
Spectrometry (MS)-based methods.
Although cell-based assays can be sensitive enough for the determination of dioxins and related substances in agreement with
the presently tolerable limits in food and feed (Regulation No. 2375/2001/EC and Directive 2003/57/EC respectively), their
lack of specificity make their use rather questionable in control laboratories.
In this paper, we present and compare results obtained from the analysis of a limited number of feed samples by both gas chromatography-high
resolution mass spectrometry (GC-HRMS) and cell-based assay (DR-CALUX: dioxin responsive-chemically activated luciferase gene
expression) methods. The DR-CALUX screening led to less than 10% false non-compliant and no false compliant results. In addition,
there is a good correlation between GC-HRMS and DR-CALUX data. However, these preliminary results have to be confirmed on
a larger number of samples to demonstrate that total toxic equivalent (TEQ), including dioxins, furans and dioxin-like polychlorobiphenyls
(PCBs) can be monitored in feed and food with a cell-based assay.
Presented at AOAC Europe/Eurachem Symposium March 2005, Brussels, Belgium |
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Keywords: | Dioxin responsive-chemically activated luciferase gene expression (DR-CALUX) Polychlorinated dibenzo-p-dioxins (PCDDs) Polychlorinated dibenzofurans (PCDFs) Polychlorinated biphenyls (PCBs) Screening Dioxin Feed Food |
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