Interdigitated electrode design and optimization for dielectrophoresis cell separation actuators |
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| Institution: | 1. Metrology and Advanced Mechatronics Laboratory, Department of Mechanical Engineering, Tarbiat Modares University, Tehran, Iran;2. Hematology Laboratory, Department of Medical Sciences, Tarbiat Modares University, Tehran, Iran;1. Institut de Microelectrònica de Barcelona, IMB-CNM (CSIC), Campus UAB, 08193 Barcelona, Spain;2. Centro de Investigacion Biomédica en Red, Biomateriales y Nanomedicina (CIBER-BBN), Spain;3. ITMO University, Kronverkskiy pr, 49, 197101, St. Petersburg, Russia;1. Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA;2. Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA;1. Department of Bio & Nano-Bio Engineering, Incheon National University, 119 Academy-ro, Incheon 22012, Republic of Korea;2. Department of Nano-Bioengineering, College of Life Sciences and Bioengineering, Incheon National University, 119 Academy-ro, Incheon 22012, Republic of Korea;1. School of Engineering and Sciences, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, NL, 64849, Mexico;2. Department of Mechanical and Aerospace Engineering, University of California, Irvine, 4200 Engineering Gateway, Irvine, CA, 92697, USA;3. BBB Inc., 551 Seolleung-ro, Yeoksam 1-dong, Gangnam-gu, Seoul, 06145, Republic of Korea;1. Institute for Nanoelectronics, Technische Universität München, DE-80333 Munich, Germany;2. ECsens, Departamento de Química Analítica, Facultad de Ciencias Universidad de Granada, E-18071 Granada, Spain;3. ECsens, Departamento de Electrónica y Tecnología de Computadores, ETSIIT Universidad de Granada, E-18071, Granada, Spain |
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| Abstract: | Among all particle separation approaches, dielectrophoresis actuators which use electric properties difference between particles, have turned into strong separating tools. This way, the particles in the fluid within non-uniform electric field experience the dielectrophoresis force. The amount and direction of this force depend on the fluid and particle polarization, particle size and electric field gradient. In this paper after presenting governing equations concerning the dielectrophoresis phenomenon, a microfluidic actuator introduced in which an interdigitated electrode pattern is applied in. Voltage, pitch, and width to pitch ratio of electrode as well as channel height are of the most important geometrical parameters of this actuator whose individual effect on particles separation was investigated using finite element analysis (FEM). The simulation results showed that if the actuator is intended to work in the efficient conditions, channel height and electrodes pitch should be near to each other, height needs to be as minimum as possible while voltage as maximum as possible in order to reach to the least time duration and the highest quantity for particles separation. Then, using theoretical equations and simulation results, a flowchart is introduced to design and optimize dielectrophoresis separation actuators. Finally, experimental results for k562 cell separation, as a biological particle, from Polystyrene, as a standard particle, is presented. In the fabricated actuator recovery and purity efficiency are 93% and about 100% respectively. |
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| Keywords: | Dielectrophoresis Particle separation Electrostatic Optimization Interdigitated electrode |
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