Pneumococcal Predictive Proteins Selected by Microbial Genomic Approach Are Serotype Cross-Reactive and Bind to Host Extracellular Matrix Proteins |
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Authors: | Ana Paula Corrêa Argondizzo Cláudio Marcos Rocha-de-Souza Marta de Almeida Santiago Ricardo Galler Joice Neves Reis Marco Alberto Medeiros |
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Affiliation: | 1.Laboratory of Recombinant Technology, Bio-Manguinhos, Brazilian Health Ministry,FIOCRUZ,Rio de Janeiro,Brazil;2.Research Laboratory of Hospital Infection, Collection Hospital Origin bacteria cultures, Instituto Oswaldo Cruz, Brazilian Health Ministry,FIOCRUZ,Rio de Janeiro,Brazil;3.Laboratory of Diagnostic Technology, Bio-Manguinhos, Brazilian Health Ministry,FIOCRUZ,Rio de Janeiro,Brazil;4.Fiocruz, Bio-Manguinhos,Brazilian Health Ministry,Rio de Janeiro,Brazil;5.School of Pharmacy,Federal University of Bahia,Salvador,Brazil |
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Abstract: | Streptococcus pneumoniae is a colonizer of the human nasopharynx, which accounts for most of the community-acquired pneumonia cases and can cause non-invasive and invasive diseases. Current available vaccines are serotype-specific and the use of recombinant proteins associated with virulence is an alternative to compose vaccines and to overcome these problems. In a previous work, we describe the identification of proteins in S. pneumoniae by reverse vaccinology and the genetic diversity of these proteins in clinical isolates. It was possible to purify a half of 20 selected proteins in soluble form. The expression of these proteins on the pneumococcal cells surface was confirmed by flow cytometry. We demonstrated that some of these proteins were able to bind to extracellular matrix proteins and were recognized by sera from patients with pneumococcal meningitis infection caused by several pneumococcal serotypes. In this context, our results suggest that these proteins may play a role in pneumococcal pathogenesis and might be considered as potential vaccine candidates. |
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