Evaluation of Chitosan-Based Films Containing Gelatin,Chondroitin 4-Sulfate and ZnO for Wound Healing |
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Authors: | Thiago B Cahú Rodrigo A Silva Raquel P F Silva Milena M Silva Isabel R S Arruda Janilson F Silva Romero M P B Costa Suzan D Santos Helena B Nader Ranilson S Bezerra |
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Institution: | 1.Department of Biochemistry,Federal University of Pernambuco (UFPE),Recife,Brazil;2.Department of Morphology, Division of Histology, School of Dentistry at Piracicaba,University of Campinas (UNICAMP),Piracicaba,Brazil;3.Department of Molecular Biology,Federal University of S?o Paulo (UNIFESP),S?o Paulo,Brazil |
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Abstract: | In this work, chitosan-based films containing gelatin and chondroitin-4-sulfate (C4S) with and without ZnO particles were produced and tested in vitro to investigate their potential wound healing properties. Chitosans were produced from shrimp-head processing waste by alkaline deacetylation of chitin to obtain chitosans differing in molecular weight and degree of deacetylation (80 ± 0.5%). The film-forming solutions (chitosan, C4S and gelatin) and ZnO suspension showed no toxicity towards fibroblasts or keratinocytes. Chitosan was able to agglutinate red blood cells, and film-forming solutions induced no hemolysis. Film components were released into solution when incubated in PBS as demonstrated by protein and sugar determination. These data suggest that a stable, chitosan-based film with low toxicity and an ability to release components would be able to establish a biocompatible microenvironment for cell growth. Chitosan-based films significantly increased the percentage of wound healing (wound contraction from 65 to 86%) in skin with full-thickness excision when compared with control (51%), after 6 days. Moreover, histological analysis showed increased granulation tissue in chitosan and chitosan/gelatin/C4S/ZnO films. Chitosan-based biopolymer composites could be used for improved biomedical applications such as wound dressings, giving them enhanced properties. |
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