Direct Ethanol Production from Ionic Liquid-Pretreated Lignocellulosic Biomass by Cellulase-Displaying Yeasts |
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| Authors: | Ryosuke Yamada Kazunori Nakashima Nanami Asai-Nakashima Wataru Tokuhara Nobuhiro Ishida Satoshi Katahira Noriho Kamiya Chiaki Ogino Akihiko Kondo |
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| Affiliation: | 1.Organization of Advanced Science and Technology,Kobe University,Kobe,Japan;2.Department of Chemical Engineering,Osaka Prefecture University,Osaka,Japan;3.Division of Sustainable Resources Engineering, Faculty of Engineering,Hokkaido University,Sapporo,Japan;4.Bio Fuel Group, Organic Material Engineering Division,Toyota Motor Corporation,Aichi,Japan;5.Biotechnology Laboratory, Toyota Central R&D Labs., Inc.,Aichi,Japan;6.Center for Future Chemistry,Kyushu University,Fukuoka,Japan;7.Department of Applied Chemistry, Graduate School of Engineering,Kyushu University,Fukuoka,Japan;8.Department of Chemical Science and Engineering, Graduate School of Engineering,Kobe University,Kobe,Japan |
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| Abstract: | Among the many types of lignocellulosic biomass pretreatment methods, the use of ionic liquids (ILs) is regarded as one of the most promising strategies. In this study, the effects of four kinds of ILs for pretreatment of lignocellulosic biomass such as bagasse, eucalyptus, and cedar were evaluated. In direct ethanol fermentation from biomass incorporated with ILs by cellulase-displaying yeast, 1-butyl-3-methylimidazolium acetate ([Bmim][OAc]) was the most effective IL. The ethanol production and yield from [Bmim][OAc]-pretreated bagasse reached 0.81 g/L and 73.4% of the theoretical yield after fermentation for 96 h. The results prove the initial concept, in which the direct fermentation from lignocellulosic biomass effectively promoted by the pretreatment with IL. |
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