Identification and High-level Production of Pulcherrimin in <Emphasis Type="Italic">Bacillus licheniformis</Emphasis> DW2 |
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Authors: | Xiaoyun Li Dong Wang Dongbo Cai Yangyang Zhan Qin Wang Shouwen Chen |
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Institution: | 1.Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, College of Life Sciences,Hubei University,Wuhan,People’s Republic of China;2.State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology,Huazhong Agricultural University,Wuhan,People’s Republic of China |
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Abstract: | Pulcherrimin, a potential biocontrol agent produced by microorganisms, has the promising applications in the agricultural, medical, and food areas, and the low yield of pulcherrimin has hindered its applications. In this study, the red pigment produced by Bacillus licheniformis DW2 was identified as pulcherrimin through the spectrometry analysis and genetic manipulation, and the component of the medium used for pulcherrimin production was optimized. Based on our results, the addition of 1.0 g L?1 Tween 80 could improve the yield of pulcherrimin, and glucose and (NH4)2SO4 were served as the optimal carbon and nitrogen sources for pulcherrimin synthesis, respectively. Furthermore, an orthogonal array design was applied for optimization of the medium. Under optimized condition, the maximum yield of pulcherrimin was 331.17 mg L?1, 5.30-fold higher than that of the initial condition, which was the maximum yield reported for pulcherrimin production. Collectively, this study provided a promising strain and a feasible approach to achieve the high-level production of antimicrobial pulcherrimin. |
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