Estimation of urinary diamines and polyamines by thin-layer chromatography |
| |
Authors: | Joseph H Fleisher Diane H Russell |
| |
Institution: | Department of Pharmacology, Arizona Medical Center, Tucson, Ariz, 85724 U.S.A. |
| |
Abstract: | The dansylated derivatives of ammonia, 1.3-diaminopropane, 1,4-diaminobutane, 1,5-diaminopentane, spermidine, histamine, and spermine were separated by one ascending development in chloroform—triethylamine (5:1) on a 250-μm silica gel 60 plate. Putrescine, cadaverine, spermidine, and spermine in human urine were quantitated by a direct scan of the fluorescent intensity of the spots corresponding to these compounds. Higher amounts of spermidine and spermine were found in the urines of cancer patients compared to the values of these substances in normal urine. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|