Comparative study of different gels for quality control of99mTc-radiopharmaceutical kits

The available six types of Sephadex and one type of Sepharose have been applied in the separation of technetium fractions in^99mTc-labelled radiopharmaceuticals produced in our laboratory using the GCS technique. By this technique the chemical state and the percentage of^99mTc-fractions have been determined. The resolution efficiency of some gel types were found to be significantly influenced by the pH of the eluent. The results obtained from the experiments indicated that Sephadex G-25 Fine was the best and can be routinely used in the radiochemical analysis of the following kits:^99mTc-HSA,^99mTc-DTPA and^99mTc-HIDA and G-100 for^99mTc-PYP. With^99mTc-HSA and^99mTc-PYP kits, 0.9% NaCl eluents at pH 3.2 and pH 2 to 2.5, respectively, were found to be necessary for the separation of^99mTc-fractions. G-50 Fine was found to be the best gel between the others in the separation of^99mTc-fractions in testing of the weak radiopharmaceuticals,^99mTc-GH and^99mTc-MDP. The development of^99mTc-MDP with the eluent at the same pH as the preparation gives negligible interaction effect.