| 固相时间分辨荧光免疫标记技术研究 |
| |
| 引用本文： | 潘利华,周誓红,孙文伟,谢文兵,赵超. 固相时间分辨荧光免疫标记技术研究[J]. 光谱学与光谱分析, 2004, 24(12): 1601-1604 |
| |
| 作者姓名： | 潘利华 周誓红 孙文伟 谢文兵 赵超 |
| |
| 作者单位： | 中国科学院长春应用化学研究所,国家电化学光谱分析研究中心,中国科学院稀土化学和物理开放实验室,吉林,长春,130022;吉林大学中日联谊医院,吉林,长春,130031 |
| |
| 基金项目： | 国家自然科学基金 (30 0 70 71 4 )资助项目 |
| |
| 摘 要： | 通过固相时间分辨荧光免疫分析双功能螯合剂4，7-二氯磺基苯-1，10菲罗啉-2，9-二羧酸标记抗-乙型肝炎表面抗体(HBsAb)IgG实验，对于BCPDA标记蛋白质的方法进行了研究。结果表明：BCPDA在相对温和条件下能与蛋白质反应，反应后蛋白质的相对生物活性高于78％，标记比为23～55，蛋白回收率达60％以上。在一定条件下与铕离子形成稳定的BCPDA-Eu^3 (HBsAb)IgG标记物。利用自建的分析方法，测定了标记过程的有关参数。并对标记物的某些光学特性进行了研究。
|
| 关 键 词： | 固相时间分辨荧光免疫 铕标记 抗-乙型肝炎表面抗体 4 7-二氯磺基苯-1 10菲罗啉-2 9-二羧酸 |
| 文章编号： | 1000-0593(2004)12-1601-04 |
| 修稿时间： | 2003-02-08 |
Study of Solid-Phase Time-Resolved Fluorescence Label Immunoassay |
| |
| PAN Li-hua ,ZHOU Shi-hong ,SUN Wen-wei ,XIE Wen-bing ,ZHAO Chao . National Analytical Research Center of Electrochemistry and Spectroscopy,Key Laboratory of Rare Earth Chemistry and Physics,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun ,China . China-Japan Union Hospital of Jilin University,Changchun ,China. Study of Solid-Phase Time-Resolved Fluorescence Label Immunoassay[J]. Spectroscopy and Spectral Analysis, 2004, 24(12): 1601-1604 |
| |
| Authors: | PAN Li-hua ZHOU Shi-hong SUN Wen-wei XIE Wen-bing ZHAO Chao . National Analytical Research Center of Electrochemistry Spectroscopy Key Laboratory of Rare Earth Chemistry Physics Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun China . China-Japan Union Hospital of Jilin University Changchun China |
| |
| Affiliation: | National Analytical Research Center of Electrochemistry and Spectroscopy, Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. |
| |
| Abstract: | This paper describes optimal conditions for HBsAbIgG labeling with a new fluorescence probe, 4,7-bis-chorosulfophenyl-1,10-phenanthroline-2,9-dicarboxylic acid(BCPDA)for the solid phase time-resolved fluorimmunoassay (TRFIA). The result of experiment under states clearly that BCPDA may react with protein under relative mild condition. The relative bioactivity of reacted protein was more than 80%. The labeling molar ratio of BCPDA for HBsAbIgG was 45-70. The recovery was higher than 80%. Protein-BCPDA-Eu 3+ complex is stable. It can emit very high fluorescence intensity with very long fluorescence life times. The fluorescence of Protein-BCPDA-Eu 3+ complex has a very large stokes shift (270 nm). The emission band at 611.2 nm is very narrow. The research provides the base for developing non-isotopic immunoassay technique and clinical medical diagnosis. |
| |
| Keywords: | Solid-phase time-resolved fluorimmunoassay Europium labels Anti-hepatits B surface 4 7-bis-chorosulfophenyl-1 10-phenanthroline-2 9-dicarboxylic acid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
