酸性深蓝5R的极谱分析方法及其与环糊精的超分子体系研究

建立并研究了酸性深蓝 5R(AC5R)的极谱分析法.结果表明,在pH=7. 0的Na2HPO4 KH2PO4底液中,AC5R产生一峰形好、稳定、灵敏的还原峰,其峰电位EP约 -0. 60V(vs.SCE),峰电流及二阶导数极谱峰高随AC5R浓度变化的线性范围在 3. 0×10-8 ~6. 0×10-5 mol/L(r=0. 992 6 ~0. 999 1 ),检测限为 6. 0×10-9mol/L.RSD为 1. 16% (n=10),标准回收率在 95. 85% ~103. 3%之间,可望用于AC5R的定量分析.此外,还应用极谱伏安法研究了各种环糊精对AC5R电化学行为的影响和超分子体系;利用电流法测定了AC5R与α CD、β CD、γ CD、DM β CD、TM β CD、HP γ CD的包结常数和包结比,比较AC5R与不同类型环糊精的包结能力,并初步研究影响包结能力大小的可能因素.

Polarographic Method of Acid Cyanine 5R and the Supramolecular System of Acid Cyanine 5R with Cyclodextrins by Polarography

The polarographic method was employed to investigate the supermolecular system of acid cyanine 5R and with cyclodextrins. It was shown that the reducing peak formed by AC 5R in Na_2HPO_4-KH_2PO_4 solution at pH=7.0 was well-defined, stable and sensitive with a peak potential near -0.60 V (vs. SCE). Variations of AC 5R concentrations with the peak currents and the peak height of second derivative polarographs fell in the linear regions of 3×10~(-8)～6×10~(-5) mol/L (r=0.9926～0.9991). The determining limit was 6×10~(-9) mol/L, RSD was 1.16%(n=10) and the standard recovery was 95.9%～103.3%. The polarographic method established is capable of application in the quantitative analyses of AC 5R. In addition, the effects of various cyclodextrins on the electrochemical behavior of AC 5R were also studied. The inclusion constants were calculated by electric current method, and possible factors which influence the inclusion of AC 5R and various of cyclodentrins are discussed.