| 一株内生真菌的原生质体制备及再生过程研究 |
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| 引用本文： | 郭丹钊,李兰. 一株内生真菌的原生质体制备及再生过程研究[J]. 河南师范大学学报(自然科学版), 2006, 34(2): 106-109 |
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| 作者姓名： | 郭丹钊 李兰 |
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| 作者单位： | 河南科技学院,生命科技学院,河南,新乡,453003;河南科技学院,生命科技学院,河南,新乡,453003 |
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| 基金项目： | 河南科技学院校科研和教改项目 |
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| 摘 要： | 通过对酶系组成和酶解条件的研究,得出了制备禾本科植物内生真菌Neotyphodiumsp.原生质体的最佳条件:鲜菌丝由10.3%蔗糖溶液预处理,酶系组成为1.5%崩溃酶、1.0%蜗牛酶、1.0%纤维素酶和2.0%溶菌酶,酶解温度32℃,pH 6.8,酶解5 h,原生质体得率达7.3×103个/mg鲜菌丝.另外,液体再生涂布平板法结合钙离子作用可使原生质体再生率达4.72%,为不含钙离子处理组的2.5倍.
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| 关 键 词： | 原生质体 内生真菌 Neotyphodium sp. 制备 再生 |
| 文章编号： | 1000-2367(2006)02-0106-04 |
| 收稿时间： | 2005-07-07 |
| 修稿时间： | 2005-07-07 |
Protoplast Preparation and Regeneration from a Gramineous Endophyte |
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| GUO Dan-Zhao,LI Lan. Protoplast Preparation and Regeneration from a Gramineous Endophyte[J]. Journal of Henan Normal University(Natural Science), 2006, 34(2): 106-109 |
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| Authors: | GUO Dan-Zhao LI Lan |
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| Abstract: | By studying the enzyme combinations and factors of enzyme-hydrolysis,the conditions for protoplast preparation from gramineous endophyte Neotyphodium sp.were optimized.While hyphae were digested by enzyme combination(pH6.8) consisting of 1.5% driselase,1% snailase,1% celluase and 2% lysozyme for 5 h at 32 ℃,the protoplasts were obtained at the percentage of 7.3×10~3 / mg.The results showed that the protoplast regeneration rate could amount to 4.27% with liquid-solid culture method and CaCl_2 treatment,which was 2.5 times higher than that in CK. |
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| Keywords: | protoplast gramineous endophyte Neotyphodium sp. preparation regeneration |
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