光谱法研究3H-吲哚探针分子与Triton X-100胶束间的相互作用

用光谱法研究了荧光分子2－（对－己基胺基）苯基－3，3－二甲基－5－乙酯基－3H吲哚基－甲基二辛基磺化铵（A）与Triton X-100胶束间的相互作用，测定了A在不同浓度的Trtiton X-100溶液中的吸收光谱，荧光光谱和荧光寿命，实验得到了A与胶束的结合常数Ks,A在胶束中所处环境的极性及其在胶束中发生的pH效应，结果表明，A与胶束间存在较强的相互作用，A在胶束中所处环境的极性及其在胶束中发生的pH效应，结果表明：A与胶束间存在较强的相互作用；A在胶束中所处环境的极性接近于甲醇的极性，并推测出了A的不同基团在胶束中所处的不同位置，同时发现：在Triton X-100胶束中A可探测到两个位置，但当Triton X-100的浓度过大时，A只能探测到一个位置。

Study on the Interactions between a 3H-Indole Probe Molecule and Triton X-100 Micelles by Spectral Methods

The interactions between a fluorescent molecule,[2 (p hexylamino) phenyl 3,3 dimethyl 5 ethoxycarbonyl 3H indole] methyldiioctadecylammonium iodide (A),with Triton X 100 micelle are investigated by absorption and fluorescence spectra.The relatively large binding constant K s shows that strong interactions between A and micelles do exist.The polarity parameter confirms that the polarity of the environment of A in the micelle is similar to that of methanol.According to the spectral characteristics and pH effects,the various sites of different groups of A in micelles are suggested.Lifetime measurements indicate that A can recognize two sites with Triton X 100 micelle at lower surfactant concentration.However,in the Triton X 100 micelle at higher surfactant concentration,A can only recognize one site.