Effectiveness and Toxicity of Several DTPA Broadening Agents for Biological ESR Spectroscopy |
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| Institution: | 1. School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China;2. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;1. Photobiology Laboratory, System Toxicology and Health Risk Assessment Group, CSIR–Indian Institute of Toxicology Research, Post Box No. 80, M.G. Marg, Lucknow 226001, Uttar Pradesh, India;2. Developmental Toxicology Laboratory, System Toxicology and Health Risk Assessment Group, CSIR–Indian Institute of Toxicology Research,Post Box No. 80, M.G. Marg, Lucknow 226001, Uttar Pradesh, India;3. Academy of Scientific and Innovative Research, CSIR-IITR Campus, Lucknow, Uttar Pradesh, India |
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| Abstract: | The effectiveness of a standard ESR broadening agent, potassium trioxalatochromiate (CrOx), for use with the spin-label tempone, was compared to that of diethylenetriaminepentaacetic acid (DTPA) containing an ion (Gd, Cr, Mn, Fe) with a large magnetic moment. Signal attenuation, line broadening, toxicity, and cell membrane permeability were compared. As a broadening agent, CrOx was most effective, followed by Fe–DTPA. CrOx proved mildly toxic while Gd-DTPA and Fe–DTPA were virtually nontoxic. The human red blood cell membrane was tested for permeability to Fe– and Gd–DTPA and found to be impermeable to both. In situations where toxicity to cells is critical, the DTPA chelates, particularly Fe–DTPA, may prove an acceptable substitute for CrOx. |
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